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. 2021 Nov 22;31(22):5037–5045.e3. doi: 10.1016/j.cub.2021.08.072

Figure 2.

Figure 2

Phage replication of SPβ WT, ΔaimR, and ΔaimR complemented

(A) Strains Δ6 lysogenic for phages SPβ WT, ΔaimR, and ΔaimR complemented with aimRSPβ were MC induced (0.5 μg/mL), and 1 mL of each culture at different time points after induction was collected. Samples were loaded in a 0.7% agarose gel, Southern blotted, and probed for phage DNA.

(B) Strains 168 lysogenic for phages SPβ WT, ΔaimR, and ΔaimR complemented with aimRSPβ were MC induced (0.5 μg/mL), and 5 mL of each culture at different time points after induction was collected. Samples were loaded in a 0.7% agarose gel, Southern blotted, and probed for phage SPβ DNA.