Figure 6.

Titer and lysogenization of SPβ WT and yopR mutant

Strains lysogenic for phages SPβ WT and SPβ amyE::Pspank-YopR yopR::ermR were MC induced (0.5 μg/mL).

(A) The number of resulting phages were quantified using 168 Δ6 or 168 Δ6 amyE::Pspank-YopR as the recipient strain. The results are represented as PFUs mL⁻¹. The means and SDs are presented (n = 3).

(B) The number of resulting lysogens were quantified using 168 Δ6 or 168 Δ6 amyE::Pspank-YopR as the recipient strain. The results are represented as CFUs mL⁻¹. The means and SDs are presented (n = 3). An ordinary one-way ANOVA of transformed data was performed to compare mean differences between SPβ lysogen titers obtained using 168 Δ6 or 168 Δ6 amyE::Pspank-YopR recipient strains (adjusted p = 0.0171).

(C) The lysates were titered using 168 Δ6 as the recipient strain. The resulting plaque morphologies were photographed. Shown was created with BioRender.com.

See also Figure S5.