Inhibition between αβc KCs
(A) Mutually exclusive FLP-mediated expression of CD8::GFP (cyan) or CsChrimson::tdTomato (crimson) in αβc KCs.
(B) Peak hyperpolarizations of CD8::GFP-positive αβc KCs during light pulses of the indicated durations, in the absence (left) or presence (right) of FLP-mediated recombination to generate a CsChrimson-expressing αβc KC population, before (green columns) or after the addition of picrotoxin (light gray columns) or TTX (dark gray column). Two-way repeated-measures ANOVA detected significant effects of light exposure and picrotoxin on the amplitude of hyperpolarization (p = 0.0034 and p < 0.0001, respectively), while TTX had no effect (p = 0.6250; Wilcoxon test). ∗p < 0.05 in post hoc comparisons. Data are means ± SEM.
(C) Example voltage responses of a CD8::GFP-positive αβc KC during a 500-ms depolarizing current step (left) and a 200-ms light pulse (right), before and after the addition of TTX (green and black traces, respectively).
See also Figure S6.