REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal 4F3 anti-discs large	Developmental Studies Hybridoma Bank, University of Iowa	RRID: AB_528203
AlexaFluor568-conjugated goat anti-mouse IgG	Invitrogen (Thermo Fisher)	A-11031 RRID: AB_144696
Chemicals, peptides, and recombinant proteins
Biocytin	Sigma-Aldrich	B4261
AlexaFluor633-conjugated streptavidin	Invitrogen (Thermo Fisher)	S21375
Paraformaldehyde	Electron Microscopy Sciences	15713
Phosphate buffered saline tablets	Oxoid	BR0014G
Vectashield antifade mounting medium	Vector Laboratories	H-1000
4-methyl-cyclohexanol (MCH)	Sigma-Aldrich	153095
3-octanol (OCT)	Sigma-Aldrich	218405
Isopentyl acetate	Sigma-Aldrich	306967
Ethyl acetate	Sigma-Aldrich	270989
Eicosane	Sigma-Aldrich	219274
Thermoplastic wax (52°C melting point)	Agar Scientific	AGG3881
TES	Sigma-Aldrich	T5691
NaCl	Sigma-Aldrich	S7653
KCl	Sigma-Aldrich	P9333
NaHCO3	Sigma-Aldrich	S6297
NaH2PO4	Sigma-Aldrich	S8282
CaCl2	Sigma-Aldrich	21115
MgCl2	Sigma-Aldrich	M1028
Trehalose	Sigma-Aldrich	T9531
Glucose	Sigma-Aldrich	G7528
Sucrose	Sigma-Aldrich	S0389
HEPES	Sigma-Aldrich	H4034
MgATP	Sigma-Aldrich	A9187
Na3GTP	Sigma-Aldrich	G6129
EGTA	Sigma-Aldrich	E4378
all-trans retinal	Sigma-Aldrich	R2500
Dimethyl sulfoxide	Sigma-Aldrich	D2650
Tetrodotoxin citrate	Tocris	1069
Picrotoxin	Tocris	1128
Mecamylamine hydrochloride	Sigma-Aldrich	M9020
Experimental models: organisms/strains
Drosophila, Canton-S	Bloomington Drosophila Stock Center	64349
Drosophila, hsFLP	Bloomington Drosophila Stock Center42	26902
Drosophila, NP6024-GAL4	Kyoto Stock Center18	105080
Drosophila, NP7175-GAL4	Kyoto Stock Center18	114120
Drosophila, R58F02-GAL4	Bloomington Drosophila Stock Center57	39186
Drosophila, MB112C-GAL4	Bloomington Drosophila Stock Center19	68263
Drosophila, MB80C-GAL4	Bloomington Drosophila Stock Center19	68285
Drosophila, MB433B-GAL4	Bloomington Drosophila Stock Center19	68324
Drosophila, MB320C-GAL4	Bloomington Drosophila Stock Center26	68253
Drosophila, MB099C-GAL4	Bloomington Drosophila Stock Center19,26	68290
Drosophila, R12G04-LexA	Bloomington Drosophila Stock Center26	52448
Drosophila, R58E02-LexA	Bloomington Drosophila Stock Center31	52740
Drosophila, R34B02-LexA	Bloomington Drosophila Stock Center26	53631
Drosophila, UAS-CD8::GFP	Bloomington Drosophila Stock Center58	32186
Drosophila, UAS-Rdl.RNAi.8-10	Bloomington Drosophila Stock Center59	89903
Drosophila, UAS-Dcr-2	Bloomington Drosophila Stock Center60	24650
Drosophila, 10xUAS-IVS-Syn21-Chrimson::tdT-3.1 (su(Hw)attP1) “UAS-CsChrimson::tdTomato”	Gift from D. Anderson61	N/A
Drosophila, 20xUAS-IVS-CsChrimson.mVenus (attp40) “UAS-CsChrimson::mVenus”	Bloomington Drosophila Stock Center62	55135
Drosophila, lexAop-rCD2::GFP	Bloomington Drosophila Stock Center63	66544
Drosophila, 13xLexAop2-IVS-CsChrimson.mVenus (attp2) “lexAop-CsChrimson”	Bloomington Drosophila Stock Center62	55139
Drosophila, 13xLexAop-IVS-GFP-p10 (su(Hw)attP5) “lexAop-GFP”	Gift from G. Rubin64	N/A
Drosophila, 10x-UAS-FRT > -IVS-mCD8::GFP-STOP-FRT > -IVS-CsChrimson::tdTomato (attp40)	This study	N/A
Recombinant DNA
Plasmid, pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP	Addgene65	32149
Software and algorithms
MATLAB	Mathworks	https://www.mathworks.com
LabVIEW	National Instruments	https://www.ni.com
ImageJ	NIH	https://imagej.nih.gov
Signal	Cambridge Electronic Design Ltd.	http://ced.co.uk
pClamp 10	Molecular Devices	https://www.moleculardevices.com
Igor Pro	Wavemetrics	https://www.wavemetrics.com
NeuroMatic	NeuroMatic	http://neuromatic.thinkrandom.com
Python 3.7.1	Python Software Foundation	https://www.python.org
pyABF 2.2.3	Harden Technologies	https://pypi.org/project/pyabf/
dabest 0.3.1		https://pypi.org/project/dabest/
Prism	GraphPad	https://www.graphpad.com
Other
Borosilicate glass capillaries	Sutter Instruments	BF150-86-10