Extended Data Fig. 1. Transgenic FRI-GFP is functionally equivalent to endogenous FRI.
a–d, Relative expression level of FRI mRNA (a), unspliced FLC (b), spliced FLC (c) and total COOLAIR (d) in Col-0 plants with introgressive FRI (FRI) or transgenic FRI-GFP with its endogenous promoter, measured by RT–qPCR. Plants were given no cold (non-vernalization, NV) or 2 weeks of cold (2 weeks of vernalization, 2WV). Data are presented as mean ± s.e.m. of three independent biological replicates. e, f, Photographs showing flowering phenotype of Col-0 (fri), FRI-GFP and FRI plants in the warm (NV) or after 5 weeks of cold exposure (5WV). Scale bars, 5 cm