(A) Wildtype, Dectin-1 knockout, TLR-2, or Dectin-1/TLR2 double knockout C57Bl/6 mice were injected i.p. with β-glucan (1 mg) or vehicle 48 and 24 hours prior to i.p. inoculation with 108 CFU P. aeruginosa with subsequent harvest of plasma and peritoneal lavage fluid 6 hours later. (B) Core (rectal) body temperature in vehicle- or β-glucan treated mice 6 hours after i.p. P. aeruginosa.
(C) CFUs of P. aeruginosa per mL of peritoneal fluid. (D-F) Number of monocytes (D), neutrophils (E), or macrophages (F) in infected vehicle- or β-glucan-treated mice. Body temperature and clearance data shown with median. All other data shown as mean ± SEM. N = 10-15 mice per group. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 by Kruskal-Wallis test followed by Dunn’s post hoc multiple comparison test (B, C) or ANOVA with Tukey’s post-hoc multiple comparison test (D-F).