Protein was isolated from BMDM treated with β-glucan for 0.5, 1.0, or 2.0 hours. BMDM treated with 100 ng/mL LPS for 1 hour or linear glucan for 0.5, 1.0, or 2.0 hours served as positive controls. Western blot of phosphorylated IkB kinase (pIkK), total IkK (IkK), phosphorylated spleen tyrosine kinase (pSyk), and total Syk (Syk). Blots are representative of three repeated experiments.