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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: J Immunol. 2021 Oct 27;207(11):2660–2672. doi: 10.4049/jimmunol.2100339

Fig. 6. IFN-γ and IFN-λ Effects on T-bet+ B cell Differentiation via TLR7/8 (R848) and BCR Agonist Activation.

Fig. 6.

Human CD19+ magnetic bead selection was used to isolate total B cells from healthy donors (n=6) for 7 day culture with TLR7/8 agonist R848 + BCR stimulation (anti-Ig(A+M+G)), with or without IFN-λ1 or IFN-γ in the presence of BAFF and IL-21. A) Flow cytometry plots of CD11c versus T-bet gated on B cells ex vivo and on day 7 of stimulation. B) Geometric mean fluorescent intensity (MFI) of T-bet in B cells in TLR7/8 and BCR activated cultures with no IFN, IFN-λ1, IFN-γ or both (λ+γ). Points connected by line represent individual donors after IFN-λ1 treatment. C) Frequency of T-bet+ B cells. Data are represented at mean±SEM. P-values shown from paired, non-parametric Wilcoxon Signed Rank Test. * indicates p’ <0.05 after Bonferroni correction for multiple testing applied for three comparisons.