Table 3.

SPR and ELISA parameters

		Surface Plasmon Resonance				CP ELISA
Ligand	Immobilization Density (RU)	ka (1/Ms)	kd (1/s)	KD (nM)	Rmax (RU)	IC50 (nM)	95% Confidence Interval (nM)
BBK32-C High Density	2011.4	1.0 x 105	4.5 x 10−5	0.45 ± 0.00	2717.2, 2680.2	15	13 to 17
BBK32-C Low Density	604.7	8.1 x 104	6.3 x 10−5	0.78 ± 0.03	476.3, 467.6	-	-
E324A	1098.8	2.0 x 105	3.4 x 10−4	1.6 ± 0.07	441.3, 430.3	n.d.	n.d.
K327A	1087.5	9.7 x 104	7.5 x 10−3	120 ± 50	185.6, 149.3	n.d.	n.d.
K327A-T331A	938.4	3.7 x 104	2.6 x 10−2	510 ± 200	479.8, 321.6	n.d.	n.d.
L236A	764.6	2.3 x 105	3.5 x 10−5	0.15 ± 0.00	1014, 1006	5.4	4.7 to 6.2
L236A-T239A	305.0	2.4 x 105	4.3 x 10−5	0.18 ± 0.00	504.4, 500.0	5.4	4.7 to 6.2
N251A	1169.9	1.8 x 105	1.1 x 10−4	0.61 ± 0.21	282.8, 281.7	85	59 to 120
R248A	2564.4	2.2 x 105	1.3 x 10−3	5.8 ± 0.10	2576, 2559	420	280 to 630
R248A-K327A	1222.1	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
R337A	1538.8	1.1 x 105	8.8 x 10−5	0.78 ± 0.13	1070, 1041	14	11 to 18
T239A	250.7	2.6 x 105	4.4 x 10−5	0.17 ± 0.00	379.8, 377.1	4.9	4.0 to 6.2
T242A	2837.4	1.0 x 105	9.9 x 10−5	0.96 ± 0.00	3192, 3157	14	9.2 to 20.9
T242A-Y245A	2055.4	2.1 x 105	1.2 x 10−2	57 ± 0.10	2117, 2093	3600	2700 to 12000
T331A	940.3	1.4 x 105	8.2 x 10−5	0.73 ± 0.30	502.6, 516.5	4	3.6 to 4.5
Y245A	2706.6	2.8 x 105	3.5 x 10−4	1.3 ± 0.01	3334, 3310	72	44 to 120
Y323A	1058.1	1.8 x 105	1.6 x 10−4	0.87 ± 0.06	475.1, 461.0	36	21 to 56
Y323A-E324A	1496.9	1.0 x 105	4.6 x 10−2	480 ± 51	146.5, 120.5	n.d.	n.d.

n.d. not determined