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. Author manuscript; available in PMC: 2022 Nov 22.
Published in final edited form as: Curr Biol. 2021 Oct 4;31(22):4923–4934.e5. doi: 10.1016/j.cub.2021.09.010

Figure 2. Modulating Gli3 repressor (Gli3R) level determines polarity of limb axis condensation in wildtype (WT) and 5’HoxdΔ/Δ limb buds.

Figure 2.

(A) NogginLacZ staining of forming limb condensations in Gli3−/− (KO) forelimb buds compared to Gli3+/- controls ranging from time E11.25-E12.5. Postaxial polarity in appearance order is preserved in zeugopod and digits, but with late appearance of additional anterior digit condensations between d2 and d3 (denoted by *). See also Figure S2.

(B) NogginLacZ staining of forming limb condensations in 5’HoxdΔ/Δ with intact Gli3R function (+/+), and with Gli3 function removed (KO). Gli3 deletion restores postaxial polarity of condensation appearance in the 5’HoxdΔ/Δ mutant, similar to wildtype (+/+) and Gli3KO controls.

(C) NogginLacZ staining of forming limb condensations in wildtype (WT) hindlimb buds from a line carrying a conditional Cre-dependent RosaGli3R transgene allele. Embryos also expressing AP2Cre show a shift to preaxial dominance of condensation appearance, similar to that seen in 5’HoxdΔ/Δ limb buds.

Related to Figure S2.