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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Plant J. 2021 Oct 8;108(4):1162–1173. doi: 10.1111/tpj.15506

Figure 1.

Figure 1.

Functional complementation of a yeast Zn uptake mutant and expression analysis of A. thaliana ZIP genes under micronutrient deficiencies. (a) Complementation test of the Zn-uptake defective yeast mutant zrt1 zrt2. Yeast cells were transformed with empty vector pDR195 or a construct containing a ZIP cDNA. IRT1 was used as a positive control. After growth overnight, cell concentration was adjusted to OD600 = 1.0 and then serially diluted 10-, 100-, and 1000-fold. For the assay, 5 μl of each dilution were plated out onto the control or Zn-limited medium and grown for 3 days at 30 °C. (b) Quantitative real time PCR analysis of four ZIP genes in shoot and root of A. thaliana seedlings grown on control or Zn− or Fe-deficient medium. Expression is shown relative to EF1α. Error bars represent the standard error of 3 replicates. Asterisks above the bars indicate significant differences from the control growth condition by Student’s t-test (p< 0.05).