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. 2021 Nov 24;11:22852. doi: 10.1038/s41598-021-02074-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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No effect on NSF behavior following adult fluoxetine treatment in C57 PN-FLX mice, and increased latency to feed in the NSF following adult fluoxetine treatment in 129 PN-FLX mice. (A) Timeline of experiment: The window for developmental administration of fluoxetine, as well as prior phenotyping and future experiments are in faded light grey; Black and checkered gray indicate chronic fluoxetine administration in adulthood and follow up NSF and OF tests. (B) Latency to feed in the NSF in C57 mice (PNVEH: unpaired t-test, p = 0.1314; PNFLX: unpaired t-test, p = 0.6949). (C) Home cage consumption in C57 mice (PNVEH: unpaired t-test, *p = 0.0341; PNFLX: unpaired t-test, p = 0.2393) (D) Latency to feed in the home cage in C57 mice (PNVEH: unpaired t-test, p = 0.5860; PNFLX: unpaired t-test, p = 0.2044). (E) Latency to feed in NSF in 129 mice. (2-way ANOVA; interaction of PN exposure and adult FLX, F (2, 136) = 3.290, *p = 0.0402; main effect of PN exposure, F (2, 136) = 8.371, ***p = 0.0004, main effect of adult FLX, F (1, 136) = 0.6054, p = 0.4379; Bonferroni post-hoc, significant increase in latency to feed with adult FLX treatment in PN-FLX 129 mice, **p = 0.0096). (F) Amount of food consumed in the home cage in a 5-min period in 129 mice. (2-way ANOVA; interaction of PN exposure and adult FLX, F (2, 136) = 0.5200, p = 0.5957, main effect of PN exposure, F (2, 136) = 0.7566, p = 0.4712, main effect of adult FLX, F (1, 136) = 1.356, p = 0.2463). Interaction effects from the 2-way ANOVA are represented as significance lines between naïve, PNVEH, and PNFLX 129 groups, and the Bonferroni post-hoc results are depicted as significance lines within the PNFLX 129 group. Light gray squares represent adult vehicle-treated animals. Black circles and checkered bars represent adult fluoxetine-treated animals. 129 s: N = 9 naïve-VEH, 8 naïve-FLX, N = 36 PNVEH-VEH, 32 PNVEH-FLX, 27 PNFLX-VEH, 30 PNFLX-F; C57s: N = 15 PNVEH-VEH, 16 PNVEH-FLX, 17 PNFLX-VEH, 20 PNFLX-FLX. Bars represent mean. Error bars represent standard error. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.