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. 2021 Nov 24;11:22891. doi: 10.1038/s41598-021-02361-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NSCs proliferation is not affected by inflammation. (a) After incubating the NSCs with different dilutions of medium obtained from LPS-stimulated macrophages (AM) or without stimulation as a control (UM) during 96 h, proliferation was analysed by measuring Neurosphere’s diameter. Graph represents the neurosphere’s diameter measured in three independent experiments. (b) Diameter of the Neurospheres of NSCs exposed to 20% V/V of UM and AM or control during 4 days. Graph represents the neurosphere’s diameter measured in three independent experiments. ns no statistical significance. (c) Diameter of the Neurospheres of NSCs exposed to 50 ng/ml of IL-1β and/or IL-6 during 96 h. Graph represents the neurosphere’s diameter measured in three independent experiments. ns no statistical significance. (d) Representative images (× 20) of neurospheres incubated in the indicated conditions. Scale bars: 50 μm.