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. 2021 Nov 24;11:22891. doi: 10.1038/s41598-021-02361-5

Figure 2.

Figure 2

NSCs differentiation is affected by inflammation and restored by PtdCho. (a) Percentage of β-III tubulin positive cells analysed by immunocytochemistry coupled to fluorescence microscopy of NSCs exposed to 20% V/V of AM and UM in the presence or in the absence of PtdCho (50 μM) during 3 days. Graph represents the percentage of neuronal differentiation measured in five independent experiments. Data were presented as mean ± SEM. ***p < 0.001; **p < 0.01; *p < 0.05. (b) Representative images (× 40) of the immunofluorescence assays with the neuronal marker (β-III tubulin, green) nuclei (DAPI, blue). Scale bars: 40 μm. Representative images of neurons morphology under the indicated condition (× 100) βIII-tubulin (green) (c) or MAP2 (red) (d) Open arrow indicates normal neuron and white arrow indicates dystrophic neuron. Scale bars: 20 μm.