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. 2021 Nov 24;11:22891. doi: 10.1038/s41598-021-02361-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PtdCho rescues neuronal function induced by AM. (a) Quantification of neuron’s soma of cells incubated under the indicated condition. Graph represents the area of neuronal soma measured in four independent experiments. Data were presented as mean ± SEM. **p < 0.01; *p < 0.05. (b) Percentage of normal neurons (black bars) and dystrophic neurons (grey bars) after 3 days in culture under the indicated conditions *p < 0.05 (Student’s T-test). (c) βIII-tubulin signal expressed as corrected total cell fluorescence (CTCF). (d) Quantification of spine number. Values of quantification are expressed as mean ± SEM, *p < 0.05. (e) Representative images of neurons (× 100) Scale bars: 10 μm and dendritic spines (× 63) under the indicated conditions after 3 days (f) and 7 days (g) of culture. Arrowheads indicate spine. Scale bars 5 μm.