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. 2021 Nov 24;11:22891. doi: 10.1038/s41598-021-02361-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Stress affects neurons depolarization. (a) Cells were incubated under the indicated condition during 3 or 7 days and loaded with Fluo-3AM. Graph represents fluorescence as arbitrary units (a.u.) detected before and after treatment with KCl. Values are expressed as mean ± SEM ****p < 0.0001. (b) Representative images (× 63) of neurons labelled with Flio-3AM before and after KCl treatment. Scale bar 20 µm.