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. 2021 Nov 24;11:22891. doi: 10.1038/s41598-021-02361-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PtdCho restores morphology of dystrophic neurons. (a) Percentage of βIII-tubulin positive cells analysed by immunocytochemistry coupled to fluorescence microscopy of NSCs exposed to 20% V/V of AM when PtdCho was added later on, after 1 day of culture, and incubated for 3 days. Graph represents the percentage of neuronal differentiation measured in three independent experiments. Data were presented as mean ± SEM. ***p < 0.001. (b) Percentage of normal neurons (black bars) and dystrophic neurons (grey bars) after 3 days in culture under the indicated conditions. ***p < 0.001 (Student’s T-test). (c) Representative images (× 40) of neurons incubated under the indicated conditions Scale bars: 40 μm. (d) Representative images of neurons morphology under the indicated condition (× 100). Open arrow indicates normal neuron and white arrow indicates dystrophic neuron. Scale bars: 20 μm.