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. 2021 Nov 24;12:6813. doi: 10.1038/s41467-021-27057-2

Fig. 2. Effect of BAY 1895344 on NB cells.

Fig. 2

a Viability of NB cells treated with either 250 nM crizotinib (criz), 50 or 100 nM BAY 1895344 (BAY) as indicated. NB cell viability was measured after 2, 4 and 6 days for five different cell lines. ALK-addicted cell lines: CLB-BAR, CLB-GE, CLB-GAR; ALK non-addicted cell lines: SK-N-AS and IMR-32 (cell lines described in detail in Supplementary Table 1). b Apoptosis was monitored by immunoblotting for PARP cleavage (PARP*, quantified below), cleaved caspase 3 (CC3), p53, p21 and tubulin (as loading control) in NB cell lines after 24 h treatment with BAY 1895433 (50 or 100 nM). pAKT was employed as readout of ALK inhibition. 250 nM crizotinib was employed as positive control in CLB-BAR and CLB-GE cells. Data are presented as mean ± SEM. n = 3 biologically independent experiments. Student’s paired t-test, p values indicated. Source data are provided as a Source Data file.