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UBP-t1 and UBP-t2 antibodies are specific. Bacterial lysates containing the full length of UBP-t1 or UBP-t2 or the homogenates of 65-day-old rat testes were resolved by SDS-PAGE on 10% polyacrylamide gels and transferred to nitrocellulose membranes. The membranes were probed with anti-UBP-t1 N-terminal extension or UBP-t2 N-terminal extension antibody separately and then incubated with horseradish peroxidase-conjugated protein A and subjected to chemiluminescent detection.
