TABLE 1.
Determination of SSCP peak patterns of control strainsa
| Species | Strain(s) | Relative retention timeb with:
|
|||
|---|---|---|---|---|---|
| RW01 | 13B | 515 | 806R | ||
| CoNS | |||||
| S. epidermidis | ATCC 12228; N421 | 207 | 244 | 254 | 328 |
| S. hominis | ATCC 27844; N026 | 199 | 238 | 254 | 328 |
| S. haemolyticus | ATCC 29970; N473 | 199 | 238 | 254 | 328 |
| S. warneri | ATCC 27836; N042 | 202 | 232 | 254 | 327 |
| S. capitis | ATCC 27840; N1812 | 197 | 238 | 254 | 328 |
| S. auricularis | N914 | 199 | 237 | 254 | 327 |
| S. simulans | ATCC 27848; P315 | 199 | 232 | 254 | 327 |
| S. cohnii | ATCC 29974 | 204 | 235 | 254 | 328 |
| S. aureus | ATCC 25923; N005 | 199 | 238 | 254 | 328 |
| E. coli | ATCC 25922; N004 | 182 | 219 | 243 | 307 |
| Viridans group streptococci | |||||
| S. mitis | ATCC 9811; CAP95B22; N108 | 201 | 211 | 262 | 350–370 |
| S. mutans | ATCC 35668; P1743 | 196 | 214 | 259 | 328 |
| S. salivarius | ATCC 25975 | 197 | 221 | 258 | 326 |
| S. pneumoniae | ATCC 49619; N465 | 201 | 211 | 262 | 350–370 |
| E. faecalis | ATCC 51299; N047 | 195 | 202 | 260 | 327 |
| K. pneumoniae | ATCC 13883; N078 | 187 | 227 | 235 | 319 |
| Pseudomonas aeruginosa | ATCC 27853; N207 | 183 | 230 | 252 | 313 |
| E. cloacae | ATCC 23355; N138 | 187 | 227 | 235 | 319 |
| M. luteus | ATCC 49732; N142 | 205 | 235 | 242 | 321 |
| Stenotrophomonas maltophila | ATCC 13637 | 194 | 227 | 250 | 330 |
| P. acnes | ATCC 11827; P147; N125; TS178 | 182 | 226 | ||
| Streptococcus group B | ATCC 27956 | 200 | 214 | 260 | 326 |
| Streptococcus group A | ATCC 19615; N679 | 200 | 214 | 263 | 322 |
| Klebsiella oxytoca | ATCC 49131 | 195 | 216 | 234 | 318 |
| E. faecium | ATCC 35667; D-5; CAPB32; VRE 1523 | 193 | 211 | 260 | 328 |
Control strains and/or previously identified blood culture isolates were used for the determination of species-specific SSCP peak patterns of common blood culture bacterial isolates. Control strains were tested a minimum of three times, and additional strains were tested at least once. The average retention times for each of four DNA fragments upon nondenaturing electrophoresis for each run within a species determined the SSCP patterns. The table is organized in order, starting with the organisms most commonly isolated from blood cultures at our institution.
Retention time of each single-stranded PCR fragment with its associated fluorescent primer (see Materials and Methods for details about primers).