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. 2021 Sep 26;14(9):2527–2542. doi: 10.14202/vetworld.2021.2527-2542

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Copyright: © Sah^a, et al.

Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Figure-2 — Biofilm formation ability of the Pasteurella multocida isolates. (a) Mean optical density of the 22 isolates measured at 600 nm after 48 h growth at 37°C. The horizontal line represents the threshold below which indicates non-biofilm producers. Biofilms of two strong biofilm-producing isolates, (b) PM4 and (c) PM7 were further observed under scanning acoustic microscopy (SAM). The SAM micrographs demonstrated the colonizing bacterial cells after 48 h incubation, and revealed how the bacteria tend to grow in clumps (micro-colonies), and the exopolysaccharide that is covering the bacteria. Error bars represent standard deviation.