Table-1.
Primers for genotyping and pathogenic gene (virulence factors-associated genes) characterization in Pasteurella multocida strains.
| Target gene | Gene product | Description | Primer sequence (Forward/Reverse) (5’- 3’) | Product size |
|---|---|---|---|---|
| kmt1 | Pasteurella multocida specific | Identification of all Pasteurella multocida isolates | ATCCGCTATTTACCCAGTGG GCTGTAAACGAACTCGCCAC |
460 |
| 16S rRNA | 16S rRNA | P16Sf P16Sr |
AGAGTTTGATYMTGGC GYTACCTTGTTACGACTT |
1500 |
| omp87 | Outer membrane and porin proteins | Outer membrane protein 87 | ATGAAAAAACTTTTAATTGCGAGC TGACTTGCGCAGTTGCATAAC |
988 |
| toxA | Toxin | Dermonecrotic toxin | TCTTAGATGAGCGACAAGG GAATGCCACACCTCTATAG |
846 |
| Hsf-1 | Adhesins | Autotransporter adhesion | TTGAGTCGGCTGTAGAGTTCG ACTCTTTAGCAGTGGGGACAACCTC |
664 |
| ptfA | Adhesins | Fimbriae | TGTGGAATTCAGCATTTTAGTGTGTC TCATGAATTCTTATGCGCAAAATCCT GCTGG |
488 |
| pfhA | Adhesins | Filamentous haemagglutinin | AGCTGATCAAGTGGTGAAC TGGTACATTGGTGAATGCTG |
275 |
| pmHAS | Hyaluronidase | Hyaluronidase | TCAATGTTTGCGATAGTCCGTTAG TGGCGAATGATCGGTGATAGA |
430 |
| nanB | Neuraminidases | Neuraminidases | GTCCTATAAAGTGACGCCGA ACAGCAAAGGAAGACTGTCC |
554 |
| hgbA | Iron acquisition related factor | Hemoglobin binding protein | TGGCGGATAGTCATCAAG CCAAAGAACCACTACCCA |
419 |
| exbB | Iron acquisition related factor | Iron regulated and acquisition factors | TTGGCTTGTGATTGAACGC TGCAGGAATGGCGACTAA A |
283 |
| sodC | Superoxide dismutases | Superoxide dismutases | AGTTAGTAGCGGGGTTGGCA TGGTGCTGGGTGATCATCATG |
235 |