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Preparation and analysis of Q-bodies with TAMRA. (a) TNF-α binding activity of Fabs. (b) Structures of 6-TAMRA-C2-maleimide. (c) CBB-stained and fluorescence images of the Q-bodies with 6-TAMRA-C2-maleimide after SDS-PAGE; (lane 1, QB1-TMR; lane 2, QB2-TMR). (d) ELISA results for analyzing the antigen-binding activity of QB1-TMR and QB2-TMR.
