Table 1. Physicochemical Characterization of RNA-Loaded SNALPsa.
| type of RNA | size (d, nm)b | PDIb | charge at pH 7.4 (mV)b,c | charge at pH 4 (mV)b,d | EE loading efficiency (%)e |
|---|---|---|---|---|---|
| siRNA | 143.85 ± 2.39 | 0.22 ± 0.02 | +2.39 ± 0.12 | +16.02 ± 2.31 | 81.81 ± 5.57 |
| mRNA | 144.54 ± 4.22 | 0.19 ± 0.02 | +0.4 ± 3.09 | +16.58 ± 3.08 | 87.05 ± 9.37 |
| mRNA–siRNA | 149.16 ± 3.06 | 0.22 ± 0.02 | –0.45 ± 0.14 | +16.53 ± 2.20 | 93.66 ± 0.59 |
a
n = >4 SNALPs per sample.
b
Size, polydispersity, and charge were measured with Zetasizer (Malvern Instruments).
c
Measured in citrate buffer (pH 4) diluted 1:200 in deionized water.
d
Measured in phosphate buffer 0.1 M (pH 7.4)
e
The encapsulation efficiency (EE %) was measured with the RiboGreen assay.