Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 25;40:373. doi: 10.1186/s13046-021-02186-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — Decitabine activates ELP5 expression. a,b RT-qPCR and western blot analysis to detect ELP5 transcription levels (a) and protein levels (b) in NOZ and GBC-SD cells treated with decitabine (DAC) at the indicated dosage for 72 h. c Cell viability analysis for NOZ and GBC-SD cells treated with DAC at the indicated dosage for 72 h. d,e Bisulfite sequencing PCR (BSP) assays for the methylation of ELP5 promoter in NOZ and GBC-SD cells treated with DAC (1 μM for NOZ (d) and10 μM for GBC-SD (e)) or DMSO (vehicle) for 72 h. ○ indicates unmethylated CpG sites and ● indicates methylated CpG sites (left panel). The bar graphs depict the ELP5 promoter methylation rates (right panel). f MS-qPCR analysis of ELP5 promoter methylation levels in NOZ and GBC-SD cells treated with DAC (1 μM for NOZ and 10 μM for GBC-SD) or DMSO for 72 h. g,h ChIP-qPCR analysis of 5mC content (g) and PAX5 occupancy (h) in ELP5 promoter in NOZ cells treated with 1 μM DAC or DMSO for 72 h. i Chromatin accessibility of ELP5 promoter in NOZ cells treated with 1 μM DAC or DMSO for 72 h. The x axes indicate the distance from the PAX5 binding site. j Western blot for ELP5 and another two subunits of elongator complex (ELP4 and ELP3) protein levels in NOZ and GBC-SD cells treated with DAC (1 μM for NOZ and 10 μM for GBC-SD) or DMSO for 72 h. k Northern blot for the thiolated tRNA abundance in NOZ cells treated with 1 μM DAC or DMSO for 72 h. Student’s t test for statistical analysis, *P < 0.05, **P < 0.01, ***P < 0.001