Table 1. Sequence of primers used in RT-qPCR.
Gene | Primers | Sequence (5’-3’) |
---|---|---|
mtDNA | Forward | TGAGCCATCCCTTCACTAGG |
Reverse | TGAGCCGCAAATTTCAGAG | |
nuclear DNA (β-actin) | Forward | CTGCTCTTTCCCAGATGAGG |
Reverse | CCACAGCACTGTAGGGGTTT | |
HIF-1α | Forward | CTACTATGTCGCTTTCTTGG |
Reverse | GTTTCTGCTGCCTTGTATGG | |
β-actin | Forward | GGAGATTACTGCCCTGGCTCCTAGC |
Reverse | GGCCGGACTCATCGTACTCCTGCTT |