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. 2021 Jun 10;70(4):469–478. doi: 10.1538/expanim.21-0034

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©2021 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 3. — MiR-34a-5p crippled the anti-apoptotic effects of Baihui-penetrating-Qubin acupuncture in the perihemorrhagic penumbra of intracerebral hemorrhage (ICH) rats. (A) Representative images of H&E staining in the perihemorrhagic penumbra of ICH rats at 200× magnification. (B) Representative Fluoro-Jade B staining in the perihemorrhagic penumbra of ICH rats at a 400× magnification. (C) Immunofluorescence targeting TUNEL and NeuN (neuron marker) in the perihemorrhagic penumbra of ICH rats at 400× magnification. (D) Quantification of Fluoro-Jade B-positive cells in the perihemorrhagic penumbra of ICH rats. (E) Quantification of TUNEL and NeuN-positive cells in the perihemorrhagic penumbra of ICH rats. Data are reported as means ± SD (n=6) and analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. *P<0.05 vs. the indicated group.