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. 2021 Jun 10;70(4):469–478. doi: 10.1538/expanim.21-0034

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©2021 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 4. — Effects of miR-34a-5p on microglia polarization in the perihemorrhagic penumbra of intracerebral hemorrhage (ICH) rats with Baihui-penetrating-Qubin acupuncture treatment. Representative western blot for (A) CD32, (B) iNOS, (C) Arg-1, (D) CD206 and (E) IL-4 in the perihemorrhagic penumbra of ICH rats with Baihui-penetrating-Qubin acupuncture treatment. (F) IL-4 release in the perihemorrhagic penumbra of ICH rats with Baihui-penetrating-Qubin acupuncture treatment. (G) Immunofluorescence targeting CD32 and Iba-1 (microglia marker) in the perihemorrhagic penumbra of ICH rats at 400× and 800× magnification. (H) Immunofluorescence targeting CD206 and Iba-1 (microglia marker) in the perihemorrhagic penumbra of ICH rats at 400× and 800× magnification. Data are reported as means ± SD (n=6) and analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. *P<0.05 vs. the indicated group.