Fig. 2.

TdTomato expression in central nervous system and peripheral organs following intravenous administration of AAV9-CBA-Cre via retro-orbital route of newborn pups. Newborn (P0) mouse pups were intravenously injected with AAV9-CBA-Cre at dosage of 1.5 × 10¹⁴ vg/kg, via retro-orbital route. Two weeks post injection, mice were sacrificed and perfused with 4% paraformaldehyde. Tissues were cryopreserved, sectioned, and stained for tdTomato expression (red) and counterstained with DAPI (cyan). TdTomato expression is seen in the mouse brain cells (A), heart (B), kidney (C), liver (D), lung (E) and skeletal muscle (F). Low level of tdTomato expression is observed in non-injected animals (A’ to F’). All scale bars=10 µm.