Fig. 3.
TdTomato expression in central nervous system and peripheral organs following neonatal retro-orbital injection of AAV9-CBA-Cre . Postnatal day 14 (P14) mouse pups were subjected to retro-orbital injection with AAV9-CBA-Cre at dosage of 2.5 × 1013 vg/kg. Two weeks post injection, mice were sacrificed and perfused with 4% paraformaldehyde. Tissues were cryopreserved, sectioned, and stained for tdTomato expression (red) and counterstained with DAPI (cyan). TdTomato immunofluorescence is detectable in the mouse brain cells (A), heart (B), kidney (C), liver (D), lung (E) and skeletal muscle (F). TdTomato signal is lowly expressed in the non-injected animals (A’ to F’). All scale bars=10 µm.