Fig. 4.

TdTomato immunofluorescence in central nervous system and peripheral organs of adult mouse following intravenous injection of AAV9-CBA-Cre via retro-orbital route. Postnatal day 21 (P21) mouse pups were intravenously injected with AAV9-CBA-Cre at dosage of 1.5 × 10¹³ vg/kg, via retro-orbital route. Two weeks post injection, mice were sacrificed and perfused with 4% paraformaldehyde. Tissues were cryopreserved, sectioned, and stained for tdTomato expression (red) and counterstained with DAPI (cyan). TdTomato immunofluorescence is detectable in the mouse brain cells (A), heart (B), kidney (C), liver (D), lung (E) and skeletal muscle (F). Low level of tdTomato expression is detectable in non-injected animals (A’ to F’). All scale bars=10 µm.