Figure 4.

Seizure‐like activity in STXBP1 transgenic worms. Worms were exposed to 7 mg/mL pentylenetetrazol (PTZ) in Dent solution for 15 minutes, and then the number of head‐bobbing convulsions was scored over a 30‐second period. The seizure‐prone unc‐49 mutant strain was used as a positive control, whereas the wild‐type N2 was used as a negative control, as it does not undergo convulsions in response to PTZ exposure. Four of the variants (V84D, C180Y, R292H, and P335L) displayed significantly higher average convulsions per animal than worms expressing wild‐type STXBP1. Ten animals per strain were analyzed in each experiment, and three independent experiments were performed (n = 30 worms per strain in total). Data are shown as mean ± standard error of the mean (convulsion rates were compared via the Kruskal‐Wallis test with Dunnett multiple comparison correction; *P < .05). Significant P values of STXBP1 mutants are as follows: V84D (.000454), C180Y (.019696), R292H (.025307), and P335L (.010462)