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. 2021 Nov 23;219(1):e20210836. doi: 10.1084/jem.20210836

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© 2021 Khalil et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure S3. — Control for NR2F1 KO in T-HEp3 cells and on neural crest cell marker gene expression. (A) Western blot shows NR2F1 protein expression level in nuclear extracts of T-HEp3 cell lines with NT RNA or four different NR2F1 gRNAs. Arrows indicate the two cell lines that were selected for use in experiments (g2 and g4). Lamin-B1 was used as a loading control. (B) mRNA levels of indicated transcripts were measured using qPCR in DMSO- or C26-treated CAM tumors from T-HEp3 cells with NT or NR2F1 gRNA (g2). Graph shows fold difference in mRNA levels obtained by qPCR and normalized to DMSO. Data represent mean ± SEM from six to eight tumors per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by t test.