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. 2021 Nov 23;221(1):e202105092. doi: 10.1083/jcb.202105092

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© 2021 Shen et al.

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Figure S3. — LUBAC and the linear ubiquitin chains were localized at the centrosome (related to Fig. 3). (A and B) RPE-1 cells were transfected with LUBAC components and cultured in the presence of serum or serum starved for 24 h. (A) The cells were stained with Ac-tubulin (Ac-tub). Insets show zoomed-in views of the boxed regions. Scale bars, 5 µm (main image) and 1 µm (magnified region). (B) The cells stained with Ac-tub () and anti-linear ubiquitin (Ub,). Insets show zoomed-in views of the boxed regions. Scale bars, 5 µm (main image) and 1 µm (magnified region).