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. 2021 Nov 23;221(1):e202103074. doi: 10.1083/jcb.202103074

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© 2021 Yu-Kemp et al.

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Figure 4. — Ena/VASP proteins are not essential for assembling specialized actomyosin structures at the ZA. (A–F) Representative images of different time points during recovery. Control (AP4) or VASP-sequestered (FP4). *, FP4mito- or AP4mito-expressing cells. (G–K) Quantification. There was no difference between control and VASP-sequestered cells in narrowing of myosin arrays (G), actin intensity (H), apical actin polarization (I), actin bundling (J), or junction straightening (K). White arrows, locations of the zoom-in insets. In G, numbers for each time point are in Table S1. n = individual borders (H–J); AP4 = 47 (H and I), FP4 = 43 (H and I), AP4 = 47 (J), FP4 = 40 (J). mito., mitochondria. In K, representative of nine experiments with two to six borders quantified/experiment/time point. Statistical analysis performed using unpaired two-way t tests (H–J). Error bars represent mean ± SD.