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. 2021 Nov 23;221(1):e202103074. doi: 10.1083/jcb.202103074

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© 2021 Yu-Kemp et al.

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Figure 9. — Caco-2 cells also assemble extensive myosin stacks during ZA assembly. (A–F) Images at different time points of Ca recovery reveal that an extensive myosin array forms during midrecovery and localizes with a less well-organized actin network. (A) Before switch the ZA has bundled actin and sarcomeric myosin. (B) Myosin arrays overlaying disorganized actin early in recovery (yellow arrows). A subset of bicellular borders tighten up first (magenta arrows). (C and D) Tricellular and short multicellular junctions tighten up last (C, yellow arrows; D). (E and F) At the end of recovery, ZA assembly is complete.