FIGURE 1.

Duration of room temperature storage of 60% fat chow affects weight and pulmonary inflammatory response to LPS. Mice were fed either 10% fat chow or 60% fat chow for 6 weeks that had been stored at room temperature for the durations noted in the figure before being exposed to nebulized Escherichia coli LPS O111:B4 (3 mg/ml; 15 min). 24 h later, mice were weighed (a) before being euthanized and bronchoalveolar lavage performed for cell count, differentials and protein (b–e). n = 3–6 mice per group *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001 compared with groups indicated by colored stars/asterisks