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. 2021 Nov 14;11(11):3256. doi: 10.3390/ani11113256

Table 4.

Performance indicators and blood metabolites (adjusted least squares means: 4 wk intervals) of first lactation Holstein-Friesian dairy cows within three fat-to-protein ratio scores in milk during early lactation (1–20 WIM).

WIM/Item 1 n FPR score 2 s.e.m p-Value
<1.0 1.0–1.5 >1.5
Weeks 1–4 of lactation
DMI, kg/day 1639 13.4 ba 13.7 a 13.0 b 0.38 <0.001
ECM, kg/day 1603 22.0 c 25.6 b 27.4 a 0.55 <0.001
Milk fat, g/kg 1639 34.0 c 43.3 b 54.1 a 0.55 <0.001
Milk protein, g/kg 1639 36.9 a 34.5 b 33.4 c 0.36 <0.001
BCS, units 1097 2.67 2.65 2.66 0.039 0.387
BW change 3, kg/wk. 1246 −2.72 a −5.32 a −9.67 b 0.947 <0.001
lnBHB 4 845 0.38 b 0.44 b 0.55 a 0.024 <0.001
lnNEFA 4 843 0.32 c 0.39 b 0.45 a 0.029 0.001
EB 5, MJ of ME/day 1554 −6.89 a −19.3 b −39.4 c 4.712 <0.001
Weeks 5–8 of lactation
DMI, kg/day 1884 16.0 a 15.9 a 15.5 b 0.36 0.021
ECM, kg/day 1881 26.4 c 28.4 b 30.3 a 0.55 <0.001
Milk fat, g/kg 1884 32.1 c 40.2 b 49.1 a 0.38 <0.001
Milk protein, g/kg 1884 32.5 a 32.1 b 31.3 c 0.28 <0.001
BCS, units 1384 2.56 2.56 2.58 0.034 0.084
BW change, kg/wk. 1839 2.71 a 1.45 a −2.16 b 0.686 <0.001
lnBHB 1034 0.41 c 0.46 b 0.52 a 0.040 <0.001
lnNEFA 1037 0.32 b 0.34 b 0.38 a 0.030 0.009
EB, MJ of ME/day 1851 3.53 a −9.16 b −23.7 c 4.44 <0.001
Weeks 9–12 of lactation
DMI, kg/day 1889 17.1 a 17.0 a 16.1 b 0.40 <0.001
ECM, kg/day 1889 26.5 c 28.4 b 29.5 a 0.55 <0.001
Milk fat, g/kg 1889 32.9 c 40.0 b 47.5 a 0.42 <0.001
Milk protein, g/kg 1889 33.3 a 32.5 b 31.5 c 0.29 <0.001
BCS, units 1385 2.52 2.52 2.52 0.032 >0.500
BW change, kg/wk 1860 4.06 a 2.19 b −0.55 c 0.633 <0.001
lnBHB 881 0.38 b 0.43 a 0.43 a 0.020 <0.001
lnNEFA 878 0.25 0.26 0.28 0.032 >0.500
EB, MJ of ME/day 1869 14.6 a 3.74 b −13.6 c 4.17 <0.001
Weeks 13–16 of lactation
DMI, kg/day 1816 17.2 ba 17.3 a 16.8 b 0.42 0.011
ECM, kg/day 1816 26.4 c 28.2 b 29.4 a 0.54 <0.001
Milk fat, g/kg 1816 33.3 c 40.5 b 47.7 a 0.43 <0.001
Milk protein, g/kg 1816 34.0 a 33.3 b 32.2 c 0.30 <0.001
BCS, units 1358 2.51 2.51 2.51 0.030 >0.500
BW change, kg/wk 1789 3.08 2.12 0.40 0.661 0.101
lnBHB 563 0.35 b 0.43 a 0.46 a 0.024 <0.001
lnNEFA 562 0.20 0.23 0.23 0.040 0.226
EB, MJ of ME/day 1796 14.1 a 7.05 b −6.82 c 4.07 <0.001
Weeks 17–20 of lactation
DMI, kg/day 1645 17.3 17.5 17.0 0.42 0.051
ECM, kg/day 1644 26.5 c 27.7 b 28.8 a 0.65 <0.001
Milk fat, g/kg 1645 34.7 c 40.9 b 48.8 a 0.53 <0.001
Milk protein, g/kg 1645 34.5 a 33.7 b 32.5 c 0.28 <0.001
BCS, units 1296 2.53 a 2.53 a 2.48 b 0.026 0.017
BW change, kg/wk. 1628 2.45 1.74 −0.23 0.711 0.136
lnBHB 377 0.38 b 0.48 a 0.48 a 0.064 <0.001
lnNEFA 377 0.21 0.19 0.22 0.040 0.481
EB, MJ of ME/day 1637 14.3 a 10.2 a −0.92 b 3.461 <0.001

a,b,c Means within a row with different superscripts differ (p ≤ 0.05) within FPR scores; 1 weeks in milk/ stage of lactation; ECM—Energy-corrected milk; FPR—fat-to-protein ratio; 2 FPR score—fat-to-protein ratio in milk; <1.0 = ‘Low’; 1.0–1.5 = ‘Normal’; >1.5 = ‘High’; 3 BW change represents the difference between current and previous BW at each week postpartum; 4 values for blood metabolites were natural log transformed; 5 EB—energy balance estimated according to equations within ‘Feed into Milk’ [36].