FIG. 6.

Myogenin immunocytochemistry reveals that calcineurin does not alter myocyte commitment. (A) 10T1/2 cells transiently transfected with MyoD become immunoreactive for the muscle-specific marker myogenin (arrowhead, green staining). (B) Even though cain cotransfection blocks MyoD-directed MyHC expression, these cells still express myogenin, suggesting no loss of transfected cells or in their respecification to the myogenic lineage. (C) Cotransfection of MyoD and calcineurin (CnA) also does not alter the number of myogenin-positive cells. Transfected cells were shifted to DM for 6 days before being immunostained for myogenin.