Figure 3.

Ruminal protozoa and fungi composition were identified by 18S rRNA sequencing. (A) The Chao1 richness estimator and Shannon’s diversity index. (B) Partial least squares discriminant analysis (PLS-DA) plot based on the relative abundance of OTUs indicates a significantly different composition of healthy versus mastitis groups. Ellipses represent 95% confidence intervals for each group. The top 10 (C) genera and (D) species identified in cow ruminal fluid, each bar refers to an individual cow.