Table 1.
Characterization of differential ruminal metabolites in healthy and mastitic cows by HPLC-MS/MS.
| Metabolites | Mean Intensity | VIP 1 | FC 2 | p-Value | FDR 3 | KEGG Pathway 4 | |
|---|---|---|---|---|---|---|---|
| Healthy | Mastitic | ||||||
| Phenethylamine | 152891.87 | 100793.32 | 2.14 | −0.60 | 0.0002 | 0.0107 | Phenylalanine metabolism |
| Pantothenic acid | 114575.33 | 33733.69 | 1.94 | −1.76 | 0.0050 | 0.0671 | Pantothenate and CoA biosynthesis |
| Xanthurenic acid | 63550.91 | 30866.76 | 2.12 | −1.04 | 0.0024 | 0.0423 | Tryptophan metabolism |
| Harmine | 55696.01 | 31729.60 | 1.59 | −0.81 | 0.0107 | 0.0949 | Biosynthesis of alkaloids derived from shikimate pathway |
| 1-(1H-Benzo[d]imidazol-2-yl)ethan-1-ol | 42332.18 | 20576.09 | 2.28 | −1.04 | 0.0001 | 0.0068 | NA |
| N-Acetylhistamine | 25705.04 | 5040.48 | 1.79 | −2.35 | 0.0056 | 0.0689 | Histidine metabolism |
| Anacardic acid | 9944.58 | 1930.33 | 1.66 | −2.37 | 0.0032 | 0.0505 | NA |
1 VIP: Variable importance in projection. 2 FC: log2(fold change); Fold change (ratio in the log base 2 scale) between the mastiticand healthy groups. 3 FDR: False discovery rate. 4 NA indicates a metabolite not associated with the KEGG pathway.