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. 2021 Oct 25;144(22):1777–1794. doi: 10.1161/CIRCULATIONAHA.121.055313

Figure 1.

Figure 1.

LmnaHGPSrev/HGPSrev (HGPSrev) mice exhibit ubiquitous progerin expression and undetectable lamin A expression. A, CRISPR-Cas9 strategy for generating HGPSrev mice (see details in Methods and Figure S1A). Cre activity generates a Lmna “reverted” allele that causes progerin suppression and lamin A restoration. B, Representative immunofluorescence images showing progerin expression (white) and nuclei (blue) in wild-type (WT) and HGPSrev mice. Scale bar, 25 µm. C, Western blot of lamin A/C, progerin, and GAPDH in 2-month-old WT and HGPSrev mice. Six mice of each genotype were analyzed, and representative images are shown of 2 mice of each genotype. The graphs show the relative amount of progerin normalized using lamin C and GAPDH as controls. (n=3–13 WT mice; n=4–12 HGPSrev mice). Statistical analysis was performed by 2-tailed t test. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. A indicates adventitia; L, lumen; M, media; and SKM, skeletal muscle.