Table 2.

Prediction of linear and discontinuous B-cell epitopes using LBTope, ABCpred, IEDB, CBTope, and DiscoTope for spike glycoprotein

Type of prediction	Server name	Signal peptide and transmembrane topology prediction	Position of epitope
ABCpred	LBTope	Signal peptide: 1–20	20–30, 67–83, 111–136, 246–260, 268–282, 297–322, 297–411, 436–450, 490–518, 527–545, 559–573, 607–627, 653–667, 696–710, 727–747, 760–774, 807–821, 840-856, 880–894, 926–940, 943–957, 980–994, 1131–1145, 1156–1170, 1161-1175, 1164–1175, 1179–1193, 1259–1273
Linear epitope (Prediction by IEDB server)	ABCpred	Transmembrane topology prediction: out (extracellular:35–1287)	879–895, 594–610, 257–273, 11112–1128, 245–261, 1180–1196, 476–492, 307–323, 648–664, 492–508, 1247–1263, 470–486, 236–252, 1206–1222, 1058–1074, 931–947, 898–914, 1064–1080, 786–802, 525–541, 464–480, 266–282, 151–167, 1240–1256, 732–748, 406–422, 391–407, 1050–1066, 97–113, 739–755, 630–646, 415–431, 200–216, 1234–150, 1084–1100, 847–863, 604–620, 280–296, 1195–1211, 1129–1145, 70–86, 689–705, 564–580, 329–345, 347–363, 1041–1057, 803–819, 674–690, 583–599, 49–65, 320–336, 288–304, 194–210, 124–140, 829–845, 360–376, 159–176, 107–123, 994–1010, 719–735, 657–673, 501–517, 1227–1243, 941–957, 838–854, 709–725, 695–711, 374–390, 301–317, 251–267, 1146–1162
	BepiPred Linear Epitope Prediction		13–37, 59–81, 97–98, 138–154, 177–189, 206–221, 250–260, 293–296, 304–322, 329–363, 369–393, 404–426, 440–501, 516–536, 555–562, 580–583, 602–606, 616–632, 634–644, 656–666, 672–690, 695–710, 773–779, 786–800, 807–814, 828–842, 988–992, 1035–1043, 1107–1118, 1133–1172, 1203–1206, 1252–1267
	Kolaskar and Tongaonkar AntigenicityPrediction		34–41, 44–51, 53–60, 65–70, 81–87, 115–121, 125–134, 136–146, 168–174, 210–216, 223–230, 239–248, 263–270, 272–278, 288–295, 333–339, 359–371, 376–385, 430–435, 488–495, 505–527, 592–599, 607–615, 617–627, 647–653, 667–674, 687–693, 723–730, 735–741, 750–763, 781–788, 803–808, 837–843, 847–853, 858–864, 873–880, 959–966, 973–979, 1003–1011, 1030–1037, 1057–1070, 1079–1085, 1123–1132, 1174–1179, 1221–1256
	Emini surface accessibility Prediction		20–32, 35–43, 73–80, 110–115, 144–153, 179–185, 202–208, 250–255, 278–284, 314–323, 352–357, 419–428, 437–442, 455–468, 495–500, 569–581, 601–606, 627–636, 655–660, 674–685, 773–779, 786–794, 808–817, 914–920, 1068–1076, 1105–1111, 1139–1162, 1179–1186, 1202–1210, 1256–1261
	Chou and FasmanBeta–Turn Prediction		20–47, 60–85, 95–120, 132–155, 178–191, 203–223, 245–263, 289–301, 310–325, 327–343, 364–376, 378–392, 408–433, 434–455, 472–510, 520–538, 540–551, 585–597, 596–609, 631–645, 652–669, 671–686, 697–717, 737–753, 772–781, 786–816, 832–848, 902–916, 923–934, 935–946, 1032–1051, 1079–1091, 1116–1131, 1133–1149, 1152–1176, 1234–1255
	Parker Hydrophilicity Prediction		20–37, 38–47, 48–55, 66–83, 87–103, 106–118, 119–128, 131–140, 141–175, 177–191, 193–202, 203–212, 213–225, 234–240, 245–270, 276–305, 306–329, 351–377, 378–391, 401–433, 435–451, 456–489, 493–510, 520–542, 543–551, 552–560, 563–584, 586–597, 598–610, 611–623, 634–650, 652–665, 670–692, 697–715, 728–740, 742–752, 768–784, 789–801, 806–820, 833–848, 931–947, 950–961, 982–995, 1014–1049, 1051–1061, 1066–1080, 1081–1094, 1111–1131, 1133–1142, 1145–1176, 1180–1197, 1200–1211, 1245–1267
	Karplusand Schulz Flexibility Prediction		20–37, 47–57, 68–85, 91–102, 105–119, 143–159, 177–192, 193–205, 211–222, 233–242, 246–261, 267–278, 278–290, 291–304, 306–320, 321–327, 352–362, 380–392, 400–419, 421–433, 434–451, 454–473, 474–487, 495–506, 523–540, 542–561, 562–584, 596–610, 625–635, 636–648, 653–663, 672–964, 698–708, 720–738, 742–753, 770–783, 787–801, 803–819, 832–843, 850–860, 861–869, 877–888, 906–918, 919–926, 929–943, 945–960, 962–973, 979–991, 992–1014, 1024–1031, 1033–1041, 1050–1060, 1067–1080, 1082–1096, 1102–1112, 1114–1128, 1133–1175, 1177–1189, 1190–1199, 1200–1210, 1253–1272
	ELLIPRO		1113–1273, 390–528, 239–265, 329–382, 64–83, 117–190, 94–103, 784–799, 1079–1092, 207–215, 555–565, 883–897, 806–815, 477–758, 577–584, 1098–1103, 837–844, 981–987, 863–869
Conformational epitope	CBTope (Antigen regions were selected)		20–42, 66–76, 133–147, 161–182, 201–216, 257–274, 283–297, 301–325, 341–361, 371–390, 400–420, 421–443, 449–460, 471–491, 492–515, 519–515, 519–530, 551–561, 598–618, 621–643, 670–690, 736–753, 761–780, 781–790, 801–830, 900–920, 921–941, 961–980, 1020–1030, 1032–1051, 1061–1080, 1097–1110, 1120–1130, 1132–1170, 1220–1250