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. 2021 Nov 25;10(1):61. doi: 10.1007/s13721-021-00347-x

Table 4.

Physical and chemical parameters of the final multi-epitope

Number of amino acids 403
Molecular weight (KD) 42.24
Theoretical pI 8.99
Instability index 58.67
Estimated half-life 1.9 hours (mammalian reticulocytes, in vitro)
> 20 hours (yeast, in vivo)
> 10 hours (Escherichia coli, in vivo)
Aliphatic index 61.99
Grand average of hydropathicity (GRAVY)  – 0.426
Predicted Solubility upon Overexpression: INSOLUBLE with probability 0.611709