Skip to main content
. 2021 Nov 8;10(11):1361. doi: 10.3390/antibiotics10111361

Table 4.

PCR primers used for amplification of ARGs, class 1 integron, and transposon.

Types ARGs Primer Sequence Amplicon Size (bp) Reference
Tetracyclines tetC CATATCGCAATACATGCGAAAAA
AAAGCCGCGGTAAATAGCAA
78 [50]
tetO ACGGAGAGTTTATTGTATACC
TGGCGTATCTATAATGTTGAC
171 [50]
Sulfonamides Sul 1 CGCACCGGAAACATCGCTGCAC
TGAAGTTCCGCCGCAAGGCTCG
162 [51]
Sul2 TCCGGTGGAGGCCGGTATCTGG
CGGGAATGCCATCTGCCTTGAG
190 [52]
Sul3 TCCGTTCAGCGAATTGGTGCAG
TTCGTTCACGCTTTACACCAGC
127 [52]
Macrolides ermA GAAATCGGATCAGGAAAAGG
AACAGCAAACCCAAAGCTC
332 [53]
ermB GATACCGTTTACGAAATTGG
GAATCGAGACTTGAGTGTGC
364 [53]
β-lactams blaOXA-10 CGCAATTATCGGCCTAGAAACT
TTGGCTTTCCGTCCCATTT
71 [54]
blaSHV GCGAAAGCCAGCTGTCGGGC
ATTGGCGGCGCTGTTATCGC
302 [55]
blaCTX-M GGAGGCGTGACGGCTTTT
TTCAGTGCGATCCAGACGAA
63 [56]
ampC CAGCCGCTGATGAAAAAATATG
CAGCGAGCCCACTTCGA
147 [57]
Integron intI TCGTGCGTCGCCATCACA
GCTTGTTCTACGGCACGTTTGA
67 [58]
Transposon tnpA-IS4 GGGCGGGTCGATTGAAA
GTGGGCGGGATCTGCTT
108 [54]
16S rRNA V4 GTGCCAGCAGCCGCGGTAA
GGACTACAAGGGTATCTAAT
292 [59]