Figure 6.

(a–g) Glutathione (GSH) biosynthesis and GSH-assisted arsenic detoxification in the shoots of wild-type (WT) and d10 and d17 mutant plants exposed to different concentrations of sodium arsenate (Na₂AsO₄; 0, 125 and 250 μM As^V). Total GSH content (a), and relative expression of genes involved in the biosyntheses of GSH (OsGSH1 (b) and OsGSH2 (c)), phytochelatin synthase, PCS (OsPCS1 (d)), arsenate reductase, AR (OsHAC1;1 (e) and OsHAC1;2 (f)) and C-type ATP-binding cassette, ABCC (OsABCC1 (g)) were determined in the shoots of three genotypes on day 3 of As^V treatments. Represented numerical data are the means with standard errors (n = 3 biological repeats). Significant differences (p < 0.05) among the treatments are denoted by distinct alphabetical letters according to a least significant difference test. As^III, arsenite; Cys, cysteine; FW, fresh weight; Glu, glutamate; Gly, glycine; γ-ECS, γ-glutamyl cysteine synthetase; GS, glutathione synthetase, HAC, high arsenate content; PC, phytochelatin.