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RT-PCR for osteogenic gene expression for preosteoblasts in GelMA/BP hydrogels. (A) Osteonectin expression upregulated at high BP concentrations (Error bars: ±SD, ** p < 0.01). (B) Osteocalcin expression upregulated at high BP concentrations (Error bars: ±SD, * p < 0.05, ** p < 0.01, and *** p < 0.001). (C) BMP 7 expression increased at high BP concentrations (Error bars: ±SD, ** p < 0.01 and *** p < 0.001). Results were normalized to the GAPDH gene.
