Table 1.
Gene (Mouse) | Accession No. | Sense Primer (5′–3′) | Antisense Primer (5′–3′) | PCR Product (bp) |
---|---|---|---|---|
SIRT1 | NM_019812.3 | CGATGACAGAACGTCACACG | ATTGTTCGAGGATCGGTGCC | 111 |
Nrf2 | NM_010902 | AAGAATAAAGTCGCCGCCCA | AGATACAAGGTGCTGAGCCG | 170 |
NQO1 | NM_008706 | CATTGCAGTGGTTTGGGGTG | TCTGGAAAGGACCGTTGTCG | 111 |
GCLm | NM_008129 | TAAGAAGGCGGCTTGATGCT | TGTGGTGAGTCCAACTGAGC | 131 |
GCLc | NM_010295 | CTTTGGGTCGCAAGTAGGAAGC | GGGCGTCCCGTCCGTTC | 182 |
HO-1 | NM_010442 | CCTCACAGATGGCGTCACTT | TGGGGGCCAGTATTGCATTT | 200 |
NF-ĸB | AY521463.1 | AGGAAGGCAAAGCGAATCCA | TCAGAACCAAGAAGGACGCC | 102 |
iNOS | NM_010927.4 | GGTGAAGGGACTGAGCTGTTA | TGAAGAGAAACTTCCAGGGGC | 163 |
TNF-α | NM_011609.4 | GCTGTTGCCCCTGGTTATCT | ATGGAGTAGACTTCGGGCCT | 102 |
IL-1β | NM_008361.4 | GCCACCTTTTGACAGTGATGAG | AGCTTCTCCACAGCCACAAT | 186 |
IL-6 | NM_031168.2 | AGTCCTTCCTACCCCAATTTCC | GGTCTTGGTCCTTAGCCACT | 79 |
NLRP3 | NM_145827.4 | TGGGTTCTGGTCAGACACGAG | GGCGGGTAATCTTCCAAATGC | 299 |
Caspase-1 | NM_009807.2 | GGACCCTCAAGTTTTGCCCT | GCAAGACGTGTACGAGTGGT | 103 |
β-actin | NM_007393.5 | TGAGCTGCGTTTTACACCCT | GCCTTCACCGTTCCAGTTTT | 198 |