Table 1.

Bacteriophage endolysins of Gram-positive bacteria since 2019.

No.	Endolysin Name	Original Phage	Targeted Pathogens	Effective Concentration	Features of Endolysin	Reference
1	LysMR-5	S. aureus phage	S. aureus, S. epidermidis	500 μg/mL	encapsulation in alginate-chitosan nanoparticles	[59]
2	LysRODI	S. aureus phage	S. aureus	20 µg/mL	encapsulation in pH-sensitive liposomes, and effective at pH 5	[60]
3	XZ.700	S. aureus phage	S. aureus	250 µg/mL	chimeric endolysin and effective against S. aureus biofilms	[61]
4	LysSAP26	S. aureus phage SAP-26	A. baumannii, E. coli, K. pneumoniae, P. aeruginosa, S. aureus, E. faecium	5–80 µg/mL	there was 40% protection rate in A. baumannii-infected mouse model	[62]
5	Lys84	S. aureus phage qdsa002	S. aureus	10 μM	effective against biofilms	[63]
6	LysSAP33	S. aureus phage SAP33	S. aureus	/	higher activity against biofilms than LysK-like endolysin	[64]
7	S25-3	S. aureus kayvirus S25-3	S. aureus	/	genus-specific against staphylococci, particularly S. aureus	[65]
8	SAL200	S. aureus phage	S. aureus	/	effective against severe pneumonia caused by S. aureus in a lethal murine model	[66]
9	LysCSA13	S. aureus phage	S. aureus	300 nM	effective against staphylococcal biofilms on various food contact surfaces	[67]
10	Lys109	S. aureus phage	S. aureus	100 nM	chimeric endolysin	[68]
11	LysP108	S. aureus phage	S. aureus	/	/	[69]
12	ClyC	S. aureus phage	S. aureus	/	chimeric endolysin	[70]
13	HY-133	S. aureus phage	S. aureus	0.12–0.5 μg/mL	chimeric endolysin	[71]
14	LysSA11	staphylococcal phage SA11	S. aureus	/	expressed and surface-displayed in Saccharomyces cerevisiae	[72]
15	Ph28	S. epidermidis phage PH15	S. epidermidis	/	/	[73]
16	MSlys	S. pneumoniae phage MS1	S. pneumoniae	2 μM	/	[74]
17	LyJH307	Streptococcus bovis phage	S. bovis, E. faecalis, S. sanguinis	50 µg/mL	highest efficacy at pH 5.5 at 39 °C	[75]
18	LyJH307	S. bovis	S. bovis	/	as a specific modulator for rumen	[76]
19	PlyC	streptococcal C1 phage	group A, C, and E streptococci	/	recognition of Streptococcus Group A carbohydrate backbone	[77]
20	LytSD	S. avermitilis phage phiSASD1	S. avermitilis, B. subtilis, S. aureus, S. lutea, E. faecalis	10 μg/mL	/	[78]
21	lys46	B. subtilis phage	K. pneumoniae, S. Typhimurium, Proteus, E. coli	/	/	[79]
22	Ply57	broad-host-range temperate phage, Izhevsk	B. cereus group	1 μM	thermostability at 55 °C	[80]
23	LysPBC5	B. cereus phage PBC5	B. cereus	/	/	[81]
24	PlyB	B. anthracis phage vB_BanS_Bcp1	B. cereus sensu lato group species	16 µg/mL	potent bacteriolytic activity against all B. cereus sensu lato isolates	[82]
25	LysB4EAD-LysSA11	B. cereus phage B4 + S. aureus phage SA11	S. aureus, B. cereus	3.0 µM	a hybrid endolysin	[33]
26	LysPBC2	B. cereus phage PBC2	Bacillus, Listeria, Clostridium	/	harboring a B. cereus spore binding domain	[30]
27	CTP1L	C. tyrobutyricum phage ΦCTP1	C. tyrobutyricum	/	the endolysin encoding gene was introduced into the nisin producer Lactococcus lactis subsp. lactis INIA 415	[83]
28	LysCPAS15	C. perfringens phage CPAS-15	C. perfringens	45 µg/mL	C. perfringens-specific, used for pathogen detection	[84]
29	CWH	C. difficile phage phiMMP01	C. difficile	/	cell wall binding domain prevents C. difficile spore outgrowth	[85]
30	Psa	C. perfringens phage st13	C. perfringens	/	an amidase endolysin that specifically lyses C. perfringens	[86]
31	LysIME-EF1	E. faecalis phage	E. faecalis	/	a novel two-component endolysin encoded by a single gene	[87]
32	ORF28 endolysin	E. faecalis phage ϕEf11	E. faecalis	15–31 μg/mL	multifunctional lytic enzyme, effective against E. faecalis biofilm	[88]
33	Lys08	E. faecalis phage PHB08	E. faecalis	0.5–1 µg/mL	effective against E. faecalis biofilms	[89]
34	EG-LYS	E. faecalis phage	E. faecalis	0.1 mg/mL	specific to E. faecalis	[90]
35	PBEF129 endolysin	E. faecalis phage PBEF129	E. faecalis	4.8 µM	effective against biofilm	[91]
36	PM-477	Gardnerella prophage	Gardnerella	0.13–8 µg/mL	no effect on beneficial lactobacilli or other species of vaginal bacteria	[92]
37	LysKB317	Lactobacillus phage EcoSau	Acetobacter, Lactobacillus, Pediococcus, Streptococcus, Weissella	0.01–1 µM	broad activity and stability from pH 4.5–7.5 up to at least 48 h; maximum activity is observed at 50 °C up to at least 72 h	[93]
38	293 endolysin	L. monocytogenes phage vB_LmoS_293	L. monocytogenes 473 and 3099, a serotype 4b and serogroup 1/2b-3b-7	/	amidase	[94]
39	LysA	mycobacteriophage D29	M. smegmatis	/	separation of M. smegmatis from a mixed culture via the cell wall binding domain	[95]
40	LysP11	Propionibacterium phage P1.1	E. rhusiopathiae	/	binding specifically to the E. rhusiopathiae cell wall	[96]
41	PlyPl23	P. larvae phage phiIBB_Pl23	P. larvae	/	first highly specific CBD targeting exclusively P. larvae cells	[97]

Note: “/” indicates data inaccessible.