Table 1.

Optimization framework for IACT by evaluating the performance of identified targets according to four objectives.

Objectives in the outer optimization problem

1. The first objective is to measure the mortality of treated cancer cells.

2. The second objective is to obtain superior cell viability of normal cells in cancer treatment.

3. The third objective is to keep the metabolic deviation of perturbed cells as dissimilar as possible to the cancer template.

4. The fourth objective is to keep the metabolic deviation of perturbed cells as similar as possible to the normal template.

subject to the constraint-based models in the inner optimization problems

1. FBA and UFD problems for treated cancer cells.

2. FBA and UFD problems for perturbation of normal cells due to cancer treatment.